Search Results for: direct immunofluorescence

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Therapeutic Strategies in Dermatology

Bullous Pemphigoid: Key Points

…pathologists can identify antibodies along the dermal/epidermal junction. Often a linear band of IgG and C3 will be seen. Indirect immunofluorescence can be performed to identify circulating autoantibodies in the patients’ serum. Finally, enzyme…

Therapeutic Strategies in Dermatology

Pruritic Urticarial Papules and Plaques of Pregnancy: Initial Evaluation

…estationis is characterized by linear C3 at the dermoepidermal junction. Routine laboratory evaluation is normal in PUPPP. Indirect immunofluorescence, if obtained, is negative. A serum bile acid level should be obtained if intrahepatic cholestasis…

Therapeutic Strategies in Dermatology

Pemphigus Vulgaris & Paraneoplastic Pemphigus: Initial Evaluation

…for H&E, and perilesional epithelium for DIF. The latter demonstrates IgG in an intercellular “chicken wire” pattern. Indirect immunofluorescence demonstrates circulating immunoreactants that bind to monkey esophagus in vitro. Disease is also…

Therapeutic Strategies in Dermatology

Pemphigus Vulgaris & Paraneoplastic Pemphigus: Key Points

…emphigus vulgaris; however, on direct immunofluorescence, in addition to the intercellular deposition of IgG, there may be deposition of IgG along the dermoepidermal junction (linear or granular pattern) not seen with PV. Additionally, indirect

Therapeutic Strategies in Dermatology

Epidermolysis Bullosa Acquisita: Overview

…nded gradually up to once every 3 months. Complications and Undesired Consequences The major pitfall is not recognizing EBA. Direct immunofluorescence is essential in the evaluation of most bullous eruptions. Cyclosporin A has many side effects, and…

Therapeutic Strategies in Dermatology

Lupus: Key Points

…pus profundus has less epidermal and interface involvement, with the focus of inflammation in the subcutaneous fat. A direct immunofluorescence testing of the skin biopsy will reveal characteristic patterns, depending on the form of cutaneous lupus….

Therapeutic Strategies in Dermatology

Herpes Simplex: Treatment

….d. Alternative for age >12 years: ACV 400 mg b.i.d.-t.i.d ACV, acyclovir; VCV valacyclovir Initial therapy Perform viral direct immunofluorescence assay (DFA) and culture to confirm diagnosis of HSV. Culture for coexistent bacterial…

Therapeutic Strategies in Dermatology

Bullous Pemphigoid: Clinical Case

…include both biopsy of a representative skin lesion for confirmation and a biopsy of perilesional normal appearing skin for immunofluorescence Perform a thorough history including any recent medications or exposures (such as visits to a nursing…

Therapeutic Strategies in Dermatology

Pruritic Urticarial Papules and Plaques of Pregnancy: Treatment

…goid gestationis may resemble PUPPP. Pemphigoid gestationis may be associated with fetal morbidity (see above). A biopsy for direct immunofluorescence may be indicated. Allergic contact dermatitis, especially to surgical prep, etc., used on the…

Therapeutic Strategies in Dermatology

Dermatitis Herpetiformis: Clinical Case

…d crusted erosions No evidence of linear burrows or other stigmata of scabetic disease Diagnosis: DH Skin biopsies (H&E, direct immunofluorescence) are performed to confirm the diagnosis Treatment options discussed; patient opts for treatment to…

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